Chemistry and Biochemistry
 

Mechanisms of Molecular Biology

Mechanisms of Molecular Biology
Second-semester biochemistry. Nucleic acid biochemistry and molecular biology: nucleotide metabolism, chromosome and chromatin structure, DNA structure and replication, RNA transcription and gene expression, protein synthesis and regulation, eukaryotic gene systems, signal transduction.
CHEM
482
 Hours3.0 Credit, 3.0 Lecture, 0.0 Lab
 PrerequisitesCHEM 481M; or CHEM 481
 TaughtWinter
 ProgramsContaining CHEM 482
Course Outcomes: 

Draw and Explain

Draw the structure of DNA, determine the topology of a plasmid, explain the chemical sequence of events that occur during DNA replication and draw structural changes that occur due to mutation.

Determine

Determine how to perform and interpret an electrophoresis separation of DNA fragments, southern blot, restriction map, polymerase chain reaction, and a DNA sequencing reaction with necessary controls.

Design

Design a DNA cloning strategy, explain controls necessary, and write procedural plan.

Mechanism

Draw and explain the mechanism of RNA transcription and how RNA is processed, spliced, and regulated. Understand the basic mechanisms of transcriptional regulation using the example of transcription of genes necessary for cholesterol synthesis.

Techniques

Determine when and how to use techniques that involve RNA: Northern blots, quantitative PCR, microarrays, S1 nuclease and primer extension mapping of the transcriptional start with appropriate controls.

Sequence

Determine the sequence of amino acids in a protein from a series of nucleotides.

Process

Draw and explain the process of protein translation and how it is regulated.

Interpret

Determine where a protein is localized in a tissue or cell: interpret a western blot, protein fusion assays.

Interacts

Determine with what a protein interacts: Interpret gel shift assays, Chromatin Immunoprecipitation, DNA footprinting, and yeast two hybrid reporter assays.

Maintain

Maintain faith through challenges and ethical boundaries in reporting and interpreting data.