Mechanisms of Molecular Biology
Draw and Explain
Draw the structure of DNA, determine the topology of a plasmid, explain the chemical sequence of events that occur during DNA replication and draw structural changes that occur due to mutation.
Determine how to perform and interpret an electrophoresis separation of DNA fragments, southern blot, restriction map, polymerase chain reaction, and a DNA sequencing reaction with necessary controls.
Design a DNA cloning strategy, explain controls necessary, and write procedural plan.
Draw and explain the mechanism of RNA transcription and how RNA is processed, spliced, and regulated. Understand the basic mechanisms of transcriptional regulation using the example of transcription of genes necessary for cholesterol synthesis.
Determine when and how to use techniques that involve RNA: Northern blots, quantitative PCR, microarrays, S1 nuclease and primer extension mapping of the transcriptional start with appropriate controls.
Determine the sequence of amino acids in a protein from a series of nucleotides.
Draw and explain the process of protein translation and how it is regulated.
Determine where a protein is localized in a tissue or cell: interpret a western blot, protein fusion assays.
Determine with what a protein interacts: Interpret gel shift assays, Chromatin Immunoprecipitation, DNA footprinting, and yeast two hybrid reporter assays.
Maintain faith through challenges and ethical boundaries in reporting and interpreting data.