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CHEM 584

Advanced Biochemistry Methods 1

Chemistry and Biochemistry College of Physical and Mathematical Sciences

Course Description

Introduction to current biochemical research methods, techniques, and instrumentation directed toward the isolation and characterization of DNA, RNA, and proteins including, DNA cloning, protein expression, enzyme kinetics, and nucleic acid and protein ligand interactions.

When Taught

Fall

Grade Rule

Grade Rule 8: A, B, C, D, E, I (Standard grade rule)

Min

3

Fixed

3

Fixed

1

Fixed

6

Title

Design and execute experiments with nucleic acids applicable to prokaryotic systems

Learning Outcome

Apply nucleic acid techniques to isolate, purify, characterize, manipulate, amplify and express DNA and/or RNA in prokaryotic systems.

Title

Design and execute experiments with proteins derived from gene expression in prokaryotes

Learning Outcome

Characterize and manipulate proteins obtained from gene expression in prokaryotic organisms.

Title

Clearly communicate research approaches and data

Learning Outcome

Communicate in written and verbal forms the methods and results of experimentation. Provide sufficient explanation of experimental techniques and approaches to enable others to repeat experiments and verify results.

Title

Use of existing resources to address research questions

Learning Outcome

Utilize available resources (e.g., online, manufacturer's protocols) to plan, execute, and troubleshoot experimental approaches.