CHEM 584

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Advanced Biochemistry Methods 1

Chemistry and Biochemistry College of Computational, Mathematical & Physical Sciences

Course Description

Introduction to current biochemical research methods, techniques, and instrumentation directed toward the isolation and characterization of DNA, RNA, and proteins including, DNA cloning, protein expression, enzyme kinetics, and nucleic acid and protein ligand interactions.

When Taught

Fall.

Min

3

Fixed/Max

3

Fixed

1

Fixed

6

Note

Completion of Chem 601 in place of Chem 201 is satisfactory.

Learning Outcome

Apply nucleic acid techniques to isolate, purify, characterize, manipulate, amplify and express DNA and/or RNA in prokaryotic systems.

Learning Outcome

Characterize and manipulate proteins obtained from gene expression in prokaryotic organisms.

Learning Outcome

Communicate in written and verbal forms the methods and results of experimentation. Provide sufficient explanation of experimental techniques and approaches to enable others to repeat experiments and verify results.

Learning Outcome

Utilize available resources (e.g., online, manufacturer's protocols) to plan, execute, and troubleshoot experimental approaches.