CHEM 455

Critically evaluate literature references, in particular experimental reaction details including reagents, starting materials, solvent, temperature, apparatus, stoichiometry, and scale. Adapt and modify known procedures to design and execute new reactions to obtain desired products on small and moderate scales (mgg).

Monitor and evaluate the successful completion of reactions using TLC with various solvents and modes, solvent changes and co-spot interpretation.

Work up reactions using the appropriate organic and aqueous system based on polarity and acid/base properties including drying and solvent removal operations.

Perform basic purification operations using recrystallization, distillation, chromatography with proper choice of solvents, techniques, mode of operation, etc. based on the properties of the product compound.

Evaluate purity and identification of structures using hands on operation of NMR, IR, MS, and polarimetry.

Prepare sample, set up and obtain routine 1D 1H and 13C NMR spectra showing adequate signal locking, shimming, formatting, data processing, spectra interpretation with complete peak assignment.

Demonstrate understanding of NMR theory including magnetic field resonance, radio frequency pulse sequences, shift, splitting, polarization transfer, signal correlation, 2D experiments including homo and heterocorrelation spectroscopy, COSY, HSQC, NOESY, and multiple bond techniques, HMBC, TOCSY

Demonstrate understanding of MS theory including ionization techniques, EI, CI, FAB, ES, MALDI, detection techniques, fragmentation patterns of functional groups, molecular ion, base peak, isotopes, N-rule.

Demonstrate understanding of polarimetry theory including plane polarized light, interaction with chiral compounds, optical rotation and determination of enantiomeric excess.

Write clear lab reports including clear experimental detail, operation, characterization, interpretation, evaluation, and conclusions.