CHEM 482

Mechanisms of Molecular Biology

Chemistry and Biochemistry College of Physical and Mathematical Sciences

Course Description

Second-semester biochemistry. Nucleic acid biochemistry and molecular biology: nucleotide metabolism, chromosome and chromatin structure, DNA structure and replication, RNA transcription and gene expression, protein synthesis and regulation, eukaryotic gene systems, signal transduction.

When Taught

Fall

Grade Rule

Grade Rule 8: A, B, C, D, E, I (Standard grade rule)

Min

3

Fixed

3

Fixed

3

Fixed

0

Title

Maintain

Learning Outcome

Maintain faith through challenges and ethical boundaries in reporting and interpreting data.

Title

Design

Learning Outcome

Design a DNA cloning strategy, explain controls necessary, and write procedural plan.

Title

Determine

Learning Outcome

Determine how to perform and interpret an electrophoresis separation of DNA fragments, southern blot, restriction map, polymerase chain reaction, and a DNA sequencing reaction with necessary controls.

Title

Interacts

Learning Outcome

Determine with what a protein interacts: Interpret gel shift assays, Chromatin Immunoprecipitation, DNA footprinting, and yeast two hybrid reporter assays.

Title

Techniques

Learning Outcome

Determine when and how to use techniques that involve RNA: Northern blots, quantitative PCR, microarrays, S1 nuclease and primer extension mapping of the transcriptional start with appropriate controls.

Title

Interpret

Learning Outcome

Determine where a protein is localized in a tissue or cell: interpret a western blot, protein fusion assays.

Title

Mechanism

Learning Outcome

Draw and explain the mechanism of RNA transcription and how RNA is processed, spliced, and regulated. Understand the basic mechanisms of transcriptional regulation using the example of transcription of genes necessary for cholesterol synthesis.

Title

Draw and Explain

Learning Outcome

Draw the structure of DNA, determine the topology of a plasmid, explain the chemical sequence of events that occur during DNA replication and draw structural changes that occur due to mutation.

Title

Sequence

Learning Outcome

Determine the sequence of amino acids in a protein from a series of nucleotides.

Title

Process

Learning Outcome

Draw and explain the process of protein translation and how it is regulated.