MMBIO 195

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Phage Hunters: Comparative Genomics

Microbiology and Molecular Biology College of Life Sciences

Course Description

Phage genome annotation. Focus on experimental design and data analysis.

When Taught

Winter

Min

3

Fixed

3

Fixed

1

Fixed

6

Note

Freshmen or sophomore students preferred. Requires two semester commitment.

Title

Research Presentation

Learning Outcome

Prepare and deliver a research presentation (oral and poster)

Title

Research Abstract

Learning Outcome

Prepare an abstract for a presentation of research data

Title

Research Reading and Writing

Learning Outcome

Gain experience in research writing and reading of scientific papers

Title

DNA Preparation

Learning Outcome

Prepare DNA for sequencing

Title

Bioinformatic Software

Learning Outcome

Learn how to use bioinformatics software for the following:DNA sequence alignment (Consed)Primer design (Primer3)Assessing ORFs for gene calls (GBrowse, Glimmer and GeneMark)Homology assessment (BLAST and Phamerator)Annotation (Apollo)Comparative analysis (positional and functional)

Title

Archiving Samples

Learning Outcome

Archive phage samples and DNA samples (submit archived phage to HHMI)

Title

GenBank

Learning Outcome

Analyze, annotate and prepare to submit genome to GenBank (submit if possible)